✦ LIBER ✦

Enzyme channeling immunoassay: A new homogeneous enzyme immunoassay technique

✍ Scribed by David J. Litman; Teresa M. Hanlon; Edwin F. Ullman

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 568 KB
Volume: 106
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90141-4

No coin nor oath required. For personal study only.

✦ Synopsis

A homogeneous enzyme immunoassay has been developed in which an antigen and glucose-6-phosphate dehydrogenase are coimmobilized on agarose beads. Binding of hexokinase-labeled antibody to the bead-bound antigen results in an accelerated conversion of glucose, ATP, and NAD+ to 6-phosphogluconolactone, ADP, and NADH. Critical parameters affecting assay response are discussed.

📜 SIMILAR VOLUMES

Novel homogeneous enzyme immunoassay: Ch

Novel homogeneous enzyme immunoassay: Chitinase activity enhancement immunoassay (caeia)

✍ Sakari Sekine; Takeshi Watanabe; Hirosato Tanaka 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 286 KB

A novel homogeneous enzyme immunoassay has been developed that takes advantage of the observation that the activity of a bacterial chitinase could be enhanced by the binding of a ligand conjugate of a monoclonal antibody to the enzyme (L-MAbchi). The enhancement was suppressed by an antiligand antib

Current status of homogeneous enzyme imm

Current status of homogeneous enzyme immunoassays

✍ Robert M. Nakamura; Bruce A. Robbins 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 928 KB

Recent advances in homogeneous enzyme immunoassays (EIAs) are discussed. EIA methods have been developed with comparable sensitivity to those of radioirnrnunoassay (RIA). EIAs have become established in many clinical laboratories and are generally being employed for: 1) homogeneous assay of low mole

A convenient homogeneous enzyme immunoas

A convenient homogeneous enzyme immunoassay for estradiol detection

✍ May L. Chiu; Tina T.-C. Tseng; Harold G. Monbouquette 📂 Article 📅 2011 🏛 Portland Press 🌐 English ⚖ 302 KB

## Abstract A convenient homogeneous enzyme immunoassay for estradiol is described. Unlike heterogeneous immunoassays, which require time‐consuming separation steps or expensive automated systems, homogeneous immunoassays, wherein all reagents are freely suspended in bulk solution, can be simple an

Homogeneous enzyme immunoassay for macro

Homogeneous enzyme immunoassay for macromolecular antigens using avidin biotin enzyme

✍ Yoshihiro Ashihara; Isao Nishizono; Eiich Miyagawa; Yasushi Kasahara 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 244 KB

A unique homogeneous enzyme immunoassay used for the determination of haptens and macromolecular antigens has been developed. It consists of avidin-antigen conjugate, anti-ligand antibody, and biotin enzyme (propionyl-CoA carboxylase). The immunological reaction of the conjugate with antibodies spec

Homogeneous enzyme immunoassay for prote

Homogeneous enzyme immunoassay for proteins employing β-galactosidase

✍ Ian Gibbons; Carl Skold; Gerald L. Rowley; Edwin F. Ullman 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 287 KB

A homogeneous enzyme immunoassay method for proteins is described. The assay components include a protein antigen-P-galactosidase conjugate, antiprotein antibody, and a synthetic macromolecular substrate. When antibody binds to the conjugate, enzyme activity is inhibited up to 95% because of steric

Homogeneous enzyme immunoassay of diosge

Homogeneous enzyme immunoassay of diosgenin and its glycosides

✍ Manju Sarkar; Shashi B. Mahato 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 467 KB

Homogeneous enzyme immunoassay has been used as a tool for the determination of diosgenin and its glycosides in plants. Diosgenin antisera was found to inhibit the activity of diosgenin hemisuccinate-horseradish peroxidase conjugate which was reversed by the addition of free diosgenin or its glycosi