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Homogeneous enzyme immunoassay for proteins employing β-galactosidase

✍ Scribed by Ian Gibbons; Carl Skold; Gerald L. Rowley; Edwin F. Ullman

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
287 KB
Volume
102
Category
Article
ISSN
0003-2697

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✦ Synopsis

A homogeneous enzyme immunoassay method for proteins is described. The assay components include a protein antigen-P-galactosidase conjugate, antiprotein antibody, and a synthetic macromolecular substrate. When antibody binds to the conjugate, enzyme activity is inhibited up to 95% because of steric exclusion of the substrate. Free-protein antigen competes for antibody, thus preventing inhibition. The extent of inhibition is related to the concentration of protein analyte. The assay is simple to perform, highly sensitive, and is generally useful for proteins with wide structural variations.

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