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Homogeneous enzyme immunoassay for macromolecular antigens using avidin biotin enzyme

✍ Scribed by Yoshihiro Ashihara; Isao Nishizono; Eiich Miyagawa; Yasushi Kasahara

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
244 KB
Volume
1
Category
Article
ISSN
0887-8013

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✦ Synopsis

A unique homogeneous enzyme immunoassay used for the determination of haptens and macromolecular antigens has been developed. It consists of avidin-antigen conjugate, anti-ligand antibody, and biotin enzyme (propionyl-CoA carboxylase). The immunological reaction of the conjugate with antibodies specific to ligand sterically prevents enzyme inactivation -~ ~ -~ by avidin. Goat IgG and theophylline were used as model antigens. Avidin-theophylline conjugate inhibited 100% of enzyme activity. Avidin goat IgG conjugate inhibited 90% of enzyme activity.

In this method, the measurable range of theophylline was from 500 ngiml to 50 pgiml, and for goat IgG, it was 1-1000 wg/ml.

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