𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A convenient homogeneous enzyme immunoassay for estradiol detection

✍ Scribed by May L. Chiu; Tina T.-C. Tseng; Harold G. Monbouquette

Publisher
Portland Press
Year
2011
Tongue
English
Weight
302 KB
Volume
58
Category
Article
ISSN
0885-4513

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A convenient homogeneous enzyme immunoassay for estradiol is described. Unlike heterogeneous immunoassays, which require time‐consuming separation steps or expensive automated systems, homogeneous immunoassays, wherein all reagents are freely suspended in bulk solution, can be simple and fast without costly instrumentation. The key component of this assay system, an estradiol–reporter enzyme conjugate, was prepared by covalently binding β‐estradiol‐6‐(O‐carboxymethyl)oxime to glucose‐6‐phosphate dehydrogenase (G6PDH) by an N‐hydroxysuccinimide‐enhanced, carbodiimide‐mediated coupling reaction. The estradiol–G6PDH activity can be repressed up to 46% upon anti‐estradiol antibody binding. The lower detection limit of the assay is 1 nM estradiol in aqueous solution, and the standard curve is linear on logit‐log scale‐up to 6.7 µM estradiol. A detection limit of 11.5 nM in estradiol‐spiked human serum samples suggests the feasibility of applying this assay to monitor estradiol levels for the prediction and prevention of ovarian hyperstimulation syndrome.

📜 SIMILAR VOLUMES

Enzyme channeling immunoassay: A new hom
Enzyme channeling immunoassay: A new homogeneous enzyme immunoassay technique
✍ David J. Litman; Teresa M. Hanlon; Edwin F. Ullman 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 568 KB

A homogeneous enzyme immunoassay has been developed in which an antigen and glucose-6-phosphate dehydrogenase are coimmobilized on agarose beads. Binding of hexokinase-labeled antibody to the bead-bound antigen results in an accelerated conversion of glucose, ATP, and NAD+ to 6-phosphogluconolactone

Homogeneous enzyme immunoassay for macro
Homogeneous enzyme immunoassay for macromolecular antigens using avidin biotin enzyme
✍ Yoshihiro Ashihara; Isao Nishizono; Eiich Miyagawa; Yasushi Kasahara 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 244 KB

A unique homogeneous enzyme immunoassay used for the determination of haptens and macromolecular antigens has been developed. It consists of avidin-antigen conjugate, anti-ligand antibody, and biotin enzyme (propionyl-CoA carboxylase). The immunological reaction of the conjugate with antibodies spec

Homogeneous enzyme immunoassay for prote
Homogeneous enzyme immunoassay for proteins employing β-galactosidase
✍ Ian Gibbons; Carl Skold; Gerald L. Rowley; Edwin F. Ullman 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 287 KB

A homogeneous enzyme immunoassay method for proteins is described. The assay components include a protein antigen-P-galactosidase conjugate, antiprotein antibody, and a synthetic macromolecular substrate. When antibody binds to the conjugate, enzyme activity is inhibited up to 95% because of steric

Homogeneous enzyme immunoassay for macro
Homogeneous enzyme immunoassay for macromolecular antigens using hybrid antibody
✍ Yoshihiro Ashihara; Isao Nishizono; Hiromasa Suzuki; Yasushi Kasahara 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 222 KB

A new homogeneous enzyme immunoassay for the determination of macromolecular antigens has been developed based upon competitive enzyme inhibition using hybrid antibodycontaining anti-ligand and anti-enzyme inhibitory antibodies. The hybrid antibody was prepared by the reaction of anti-glucose-6-pho