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β-Galactosidase immunoassay for the measurement of cyclic AMP

✍ Scribed by Evelyne Joseph; Jean-Luc Guesdon

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
425 KB
Volume
119
Category
Article
ISSN
0003-2697

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✦ Synopsis

A specific and sensitive &galactosidase immunoassay is described. A cyclic AMP-fi-galactosidase conjugate is used as the labeled cyclic AMP derivative in a competitive binding assay. By determining the ,%galactosidase activity, the concentration of the antibody-bound conjugate is easily detected. Cyclic AMP levels and adenylate cyclase activities in Escherichia coli K12 can be determined by using this @-galactosidase immunoassay. Comparative studies performed between this enzyme immunoassay and the conventional radioimmunoassay show satisfactory correlation between the two methods.

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