Use of inactive β-d-galactosidase for elimination of interference by anti-β-d-galactosidase antibodies in immune complex transfer enzyme immunoassay for anti-thyroglobulin igg in serum using β-d-galactosidase from escherichia coli as label
✍ Scribed by Takeyuki Kohno; Dr. Eiji Ishikawa; Hiroyuki Katsumaru; Hidetaka Nakamoto; Taeko Yasuda; Tomohiro Mitsukawa; Shigeru Matsukura; Yoshizo Tsunetoshi
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 757 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0887-8013
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✦ Synopsis
A novel enzyme immunoassay (immune complex transfer enzyme immunoassay) for anti-thyroglobulin IgG using beta-D-galactosidase from Escherichia coli as label was reported previously. This immunoassay was highly sensitive in demonstrating anti-thyroglobulin IgG not only in all patients with Graves' disease and chronic thyroiditis but also in a large proportion of healthy subjects. However, the detection of anti-thyroglobulin IgG at low levels in some serum samples was difficult, probably due to the presence of anti-beta-D-galactosidase antibodies. In the present study, the use of inactive beta-D-galactosidase was tested for elimination of interference by anti-beta-D-galactosidase antibodies. Preincubation of serum samples with excess of inactive beta-D-galactosidase resulted in sufficiently low backgrounds to detect low levels of anti-thyroglobulin IgG with little effect on the dose-response of anti-thyroglobulin IgG. As a result, it was revealed that anti-thyroglobulin IgG was present in almost all healthy subjects as well as all patients with Graves' disease and chronic thyroiditis.