The measurement of cyclic GMP and cyclic AMP phosphodiesterases
โ Scribed by Joyce G. Carter; Sosamma J. Berger; Oliver H. Lowry
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 847 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
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A method has been established to purify and separate cyclic AMP and cyclic GMP from canine bile. Perchloric acid extracts are treated with Dowex 1 resin. This batch processing binds the nucleotides completely. but very little biliary pigment (less than 5%). The Dowex I resin is then layered onto a D
The fluorescent 2'-methylanthraniloyl derivative of cyclic GMP undergoes a 45% decrease in fluorescence when it is cleaved by brain phosphodiesterase in the presence of calmodulin. This fluorescence decrease is dependent upon calcium, calmodulin, and phosphodiesterase, and correlates well (r = 0.996