Novel homogeneous enzyme immunoassay: Chitinase activity enhancement immunoassay (caeia)
✍ Scribed by Sakari Sekine; Takeshi Watanabe; Hirosato Tanaka
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 286 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0887-8013
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✦ Synopsis
A novel homogeneous enzyme immunoassay has been developed that takes advantage of the observation that the activity of a bacterial chitinase could be enhanced by the binding of a ligand conjugate of a monoclonal antibody to the enzyme (L-MAbchi). The enhancement was suppressed by an antiligand antibody (AbL). Free ligand molecules competed for AbL combining with L-MAbchi, and the suppression of the enhancement was re-duced. Thus the concentration of free ligand could be estimated. Biotin was used as a ligand in this model experiment. When this monoclonal antibody was conjugated with a ligand (e.g., biotin), we furthermore found that the enhancement could be suppressed by an antiligand antibody (AbL). When free ligand molecules are added to the system, competition with L-MAbchi results to reduce the suppression of enhancement. o 1995 Wiley-Liss, Inc.