✦ LIBER ✦

Homogeneous enzyme immunoassay of diosgenin and its glycosides

✍ Scribed by Manju Sarkar; Shashi B. Mahato

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 467 KB
Volume: 136
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90319-1

No coin nor oath required. For personal study only.

✦ Synopsis

Homogeneous enzyme immunoassay has been used as a tool for the determination of diosgenin and its glycosides in plants. Diosgenin antisera was found to inhibit the activity of diosgenin hemisuccinate-horseradish peroxidase conjugate which was reversed by the addition of free diosgenin or its glycosides. The increase of enzyme activity was proportional to the quantity of the hapten over a certain range of hapten concentration. Thus, a minimum of 2.5 micrograms/ml of diosgenin and 11.5 micrograms/ml of diosgenin glycosides could be determined by this method. The results were comparable with those obtained by high-performance liquid chromatography and gravimetric methods.

📜 SIMILAR VOLUMES

Current status of homogeneous enzyme imm

Current status of homogeneous enzyme immunoassays

✍ Robert M. Nakamura; Bruce A. Robbins 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 928 KB

Recent advances in homogeneous enzyme immunoassays (EIAs) are discussed. EIA methods have been developed with comparable sensitivity to those of radioirnrnunoassay (RIA). EIAs have become established in many clinical laboratories and are generally being employed for: 1) homogeneous assay of low mole

Novel homogeneous enzyme immunoassay: Ch

Novel homogeneous enzyme immunoassay: Chitinase activity enhancement immunoassay (caeia)

✍ Sakari Sekine; Takeshi Watanabe; Hirosato Tanaka 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 286 KB

A novel homogeneous enzyme immunoassay has been developed that takes advantage of the observation that the activity of a bacterial chitinase could be enhanced by the binding of a ligand conjugate of a monoclonal antibody to the enzyme (L-MAbchi). The enhancement was suppressed by an antiligand antib

Homogeneous enzyme immunoassay for macro

Homogeneous enzyme immunoassay for macromolecular antigens using avidin biotin enzyme

✍ Yoshihiro Ashihara; Isao Nishizono; Eiich Miyagawa; Yasushi Kasahara 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 244 KB

A unique homogeneous enzyme immunoassay used for the determination of haptens and macromolecular antigens has been developed. It consists of avidin-antigen conjugate, anti-ligand antibody, and biotin enzyme (propionyl-CoA carboxylase). The immunological reaction of the conjugate with antibodies spec

Homogeneous enzyme immunoassay for macro

Homogeneous enzyme immunoassay for macromolecular antigens using hybrid antibody

✍ Yoshihiro Ashihara; Isao Nishizono; Hiromasa Suzuki; Yasushi Kasahara 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 222 KB

A new homogeneous enzyme immunoassay for the determination of macromolecular antigens has been developed based upon competitive enzyme inhibition using hybrid antibodycontaining anti-ligand and anti-enzyme inhibitory antibodies. The hybrid antibody was prepared by the reaction of anti-glucose-6-pho

Chemiluminescent assay of various enzyme

Chemiluminescent assay of various enzyme activites and its application to enzyme immunoassays

✍ Maeda, Masako ;Arakawa, Hidetoshi ;Tsuji, Akio 📂 Article 📅 1989 🏛 John Wiley and Sons ⚖ 602 KB

A highly sensitive chemiluminescent assay for NAD(P)H have been developed. The principle of the method is as follows; NAD(P)H reduces molecular oxygen t o superoxide anion (0;) and hydrogen peroxide (H202) in the presence of I-methoxy-5-methylphenazinium methyl sulphate (I-MPMS) as electron mediator

Enzyme inhibitory homogeneous immunoassa

Enzyme inhibitory homogeneous immunoassay for high molecular weight antigen (I)

✍ Yoshihiro Ashihara; Isao Nishizono; Tetsuji Tanimoto; Hiromichi Tsuchiya; Katsuh 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 375 KB

A highly sensitive homogeneous enzyme of substrate, and the enzyme reaction is immunoassay has been developed for the terminated by adding the stopper. Then determination of antigens with high molec-the mixture is centrifuged at 3,000 rpm for ular weight. The method presented is 1 minute, and the ab