𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Electrospray ionisation Fourier-transform ion cyclotron resonance mass spectrometry of dynamic combinatorial libraries

✍ Scribed by Sally-Ann Poulsen; Paul J. Gates; Graham R. L. Cousins; Jeremy K. M. Sanders

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
97 KB
Volume
14
Category
Article
ISSN
0951-4198

No coin nor oath required. For personal study only.

✦ Synopsis

The use of electrospray ionisation Fourier-transform ion cyclotron resonance tandem mass spectrometry (ESI-FTICR-MS/MS) for the analysis of dynamic combinatorial libraries (DCLs) of pseudo-peptide macrocyclic hydrazone oligomers is presented. The design of library building blocks results in mixtures of compounds with greater diversity than libraries generated by conventional combinatorial chemistry and so presents increased demands for analysis. The extended capabilities of the FTICR technique, specifically selective ion trapping, sensitivity, high resolution and mass accuracy over a broad mass range, are compatible with these increased demands and, most importantly, without the need for chromatography. Preliminary studies on the sequencing of cyclic oligomers and confirmation of the presence of sequence isomers are presented. These studies highlight the potential of FTICR-MS as a superior technique for the analysis of combinatorially generated compounds.

📜 SIMILAR VOLUMES

Assessing enzyme substrate specificity u
Assessing enzyme substrate specificity using combinatorial libraries and electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry
✍ Maria Wigger; Joseph P. Nawrocki; Clifford H. Watson; John R. Eyler; Steven A. B 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 111 KB 👁 2 views

A model experiment for the 'on-line' screening of substrate libraries by enzymes using combinatorial libraries in combination with electrospray ionization-Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry has been performed. The reaction between the electrophilic substrate 1-ch

Characterization of Combinatorial Peptid
Characterization of Combinatorial Peptide Libraries by Electrospray Ionization Fourier Transform Mass Spectrometry
✍ Brian E. Winger; Joseph E. Campana 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 275 KB 👁 2 views

The advantages of Fourier transform mass spectrometry (FTMS) are precision high mass accuracy measurements and the capability of high resolution, multistage mass spectrometry together with a number of other advanced features. These powerful facilities can be used to rapidly screen complex mixtures w

Positive-ion Electrospray–Fourier Transf
Positive-ion Electrospray–Fourier Transform Ion Cyclotron Resonance Mass Spectra of Polypeptides and Proteins
✍ Meiyu He; Jiaxi Xu; Tak Wah Dominic Chan; Rebecca L. C. Lau 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 343 KB 👁 1 views

We have determined the mas spectra of three polypeptides which have been designed as an effective reagent to be used in the diagnosis of a Chinese strain of Hepatitis E Virus (HEV). The spectra were determined by elecbrospwy ionization-Fourier transform ion cyclotron resonance mass spectrometer. The

Advantages of High Magnetic Field for Fo
Advantages of High Magnetic Field for Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
✍ Alan G. Marshall; Shenheng Guan 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 488 KB 👁 1 views

It is well-known that mass resolving power in Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) can increase linearly with increasing applied magnetic field induction, B. Here, we show that eight other FTICR primary performance parameters theoretically also increase linearly (qu

Fourier Transform Ion Cyclotron Resonanc
Fourier Transform Ion Cyclotron Resonance Mass Spectrometry in a 20 T Resistive Magnet
✍ Christopher L. Hendrickson; Jared J. Drader; David A. Laude; Shenheng Guan; Alan 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 378 KB 👁 1 views

We present Fourier transform ion cyclotron resonance (FTICR) mass spectra at a magnetic field of 20 T; more than twice the highest field previously used for FTICR. Our instrument is based on a resistive magnet installed at the National High Magnetic Field Laboratory. The magnet has a 50mm diameter b