𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Assessing enzyme substrate specificity using combinatorial libraries and electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry

✍ Scribed by Maria Wigger; Joseph P. Nawrocki; Clifford H. Watson; John R. Eyler; Steven A. Benner

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
111 KB
Volume
11
Category
Article
ISSN
0951-4198

No coin nor oath required. For personal study only.

✦ Synopsis

A model experiment for the 'on-line' screening of substrate libraries by enzymes using combinatorial libraries in combination with electrospray ionization-Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry has been performed. The reaction between the electrophilic substrate 1-chloro-2,4-dinitrobenzene and components of a H-Ξ³-Glu-Cys-Xxx-OH library, catalyzed by glutathione-S-transferase, has been monitored. It shows the feasibility of 'two-dimensional' screening of substrate libraries by ESI-FTICR mass spectrometry.

πŸ“œ SIMILAR VOLUMES

Electrospray ionisation Fourier-transfor
Electrospray ionisation Fourier-transform ion cyclotron resonance mass spectrometry of dynamic combinatorial libraries
✍ Sally-Ann Poulsen; Paul J. Gates; Graham R. L. Cousins; Jeremyβ€…K. M. Sanders πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 97 KB πŸ‘ 1 views

The use of electrospray ionisation Fourier-transform ion cyclotron resonance tandem mass spectrometry (ESI-FTICR-MS/MS) for the analysis of dynamic combinatorial libraries (DCLs) of pseudo-peptide macrocyclic hydrazone oligomers is presented. The design of library building blocks results in mixtures

Quantification of singly charged biomole
Quantification of singly charged biomolecules by electrospray ionization fourier transform ion cyclotron resonance mass spectrometry utilizing an internal standard
✍ Eric F. Gordon; David C. Muddiman πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 132 KB πŸ‘ 1 views

The quantification of Cyclosporin A (CsA) and related biological compounds by various mass spectrometric techniques is well established. It is precisely this fact that makes the use of CsA particularly appealing as a model system in evaluating the quantitative properties of electrospray ionization c

Hydropathic influences on the quantifica
Hydropathic influences on the quantification of equine heart cytochromec using relative ion abundance measurements by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
✍ Gordon, Eric F.; Mansoori, Bashir A.; Carroll, Charlotte F.; Muddiman, David C. πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 236 KB πŸ‘ 1 views

The number of publications documenting the utility of electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) for the analysis of biological molecules has increased in geometric proportion spanning diverse areas of research. Currently, we are investigatin

The use of a stable internal mass standa
The use of a stable internal mass standard for accurate mass measurements of oligonucleotide fragment ions using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry with infrared multiphoton dissociation
✍ Steven A. Hofstadler; Richard H. Griffey; Ljiljana Pasa-Tolic; Richard D. Smith πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 85 KB πŸ‘ 2 views

In this work we demonstrate the use of peptide as a stable internal mass standard to obtain accurate mass measurements of infrared multiphoton dissociation (IRMPD) generated phosphorothioate oligonucleotide fragment ions. Because oligonucleotides are more readily dissociated in the gas phase by IRMP

Accurate characterization of the tyrosin
Accurate characterization of the tyrosine hydroxylase forensic allele 9.3 through development of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
✍ James C. Hannis; David C. Muddiman πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 118 KB πŸ‘ 1 views

Accurate and precise determination of the number of repeats from a short tandem repeat (STR) sequence for a human gene locus is demonstrated for the first time by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS). Specifically, the polymorphic human t