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Positive-ion Electrospray–Fourier Transform Ion Cyclotron Resonance Mass Spectra of Polypeptides and Proteins

✍ Scribed by Meiyu He; Jiaxi Xu; Tak Wah Dominic Chan; Rebecca L. C. Lau

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
343 KB
Volume
10
Category
Article
ISSN
0951-4198

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✦ Synopsis

We have determined the mas spectra of three polypeptides which have been designed as an effective reagent to be used in the diagnosis of a Chinese strain of Hepatitis E Virus (HEV). The spectra were determined by elecbrospwy ionization-Fourier transform ion cyclotron resonance mass spectrometer. These are peptide 30, peptide 33 and peptide 42, where the guanidine amino group of arginine in each polypeptide was protected. The molecular weights were respectively measured as 3575,4094 and 5390 Dalton, against the molecular weights of bovine serum albumin (66430 Da) and bovine insulin (5732.77 Da).

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