Electrospray Ionization Fourier Transform Ion Cyclotron Resonance at 9.4 T

✍ Scribed by Michael W. Senko; Christopher L. Hendrickson; Ljiljana Paša-Tolić; Jarrod A. Marto; Forest M. White; Shenheng Guan; Alan G. Marshall

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 476 KB
• Volume: 10
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(199611)10:14<1824::aid-rcm695>3.0.co;2-e

✦ Synopsis

We present the first results from a new electrospray ionization Fourier transform ion cyclotron resonance mass spectrometer operated at a magnetic field of 9.4 T (i.e. 2 2.4 T higher than for any prior FTICR instrument). The 9.4 T instrument provides substantially improved performance for large molecules (a 50% increase in mass resolving power) and complex mixtures ( 3 100% increase in dynamic range) compared to lower-field ( c 6 T) instruments. The higher magnetic field makes possible larger trapped-ion population without introduction of significant space-charge effects such as spectral peak shift and/or distortion, and coalescence of closely-spaced resonances. For bovine ubiquitin (8.6 kDa) we obsrve accurate relative isotopic abundances at a signal-to-noise ratio greater than lOOO:l, whereas a complete nozzle-skimmer dissociation electrospray ionization (=I) FTICR mass spectrum of bovine carbonic anhydrase (29 kDa) is achieved from a single scan with a signal-to-noise ratio of more than 250:l. Finally, we are able to obtain mass resolving power, m/Am >200 OOO, routinely for porcine serum albumin (67 m a ) . The present performance guides further modifications of the instrument, which should lead to significant further improvements.