Fourier Transform Ion Cyclotron Resonance Mass Spectrometry in a 20 T Resistive Magnet

✍ Scribed by Christopher L. Hendrickson; Jared J. Drader; David A. Laude; Shenheng Guan; Alan G. Marshall

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 378 KB
• Volume: 10
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(199611)10:14<1829::aid-rcm697>3.0.co;2-u

✦ Synopsis

We present Fourier transform ion cyclotron resonance (FTICR) mass spectra at a magnetic field of 20 T; more than twice the highest field previously used for FTICR. Our instrument is based on a resistive magnet installed at the National High Magnetic Field Laboratory. The magnet has a 50mm diameter bore and spatial inhomogeneity of -lo00 ppm over a 1 cm diameter spherical volume. However, Fl'ICR mass resolving power far in excess of magnet homogeneity is achieved routinely for ions produced by either electron ionization (EI) or matrix-assisted laser desorption/ionization (MALDI). As examples, we show a MALDI mess spectrum of [M + HI' quasimolecular ions of the peptide, human luteinizing hormone-releasing hormone (monoisotopic molecular weight, 1181.6 Da) at mass resolving power, m/Am > 10 OOO, and an EI mass spectrum of molecular ions of the platinum cluster compound, Pt4(PF3), (average molecular weight, 1484 Da at mass resolving power, m/ h = 2 O OOO. Much better FTICR MS performance is predicted for future NHMFL resistive magnets of higher spatial and temporal homogeneity.