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A simple method for recovering DNA from agarose gels using glass fibre filters

✍ Scribed by Vijeshwar Verma; Gulam N. Qazi

Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
194 KB
Volume
7
Category
Article
ISSN
0951-208X

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✦ Synopsis

A simple and reproducible procedure for the recovery of plasmid DNA is described.

The method was standardised for the purification of plasmids from Giuconobacfer oxydans ATCC9937 . The protocol is based on the use of glass microfibre filter paper for entrapment of DNA and its subsequent recovery by an elution buffer. The method precludes the use of phenol and butanol for the removal of proteins and ethidium bromide respectively, therefore, making the procedure inexpensive and gentle.

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A simple technique has been developed for eluting, in high concentration, macromolecular components separated in cylindrical tubes by gel electrophoresis. The principle can also be applied to the concentration of solutions of macromolecular components obtained through other means. Soluble complement