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A Pressure–Extrusion Method for DNA Extraction from Agarose Gels

✍ Scribed by Alexander N. Gubin; Randall L. Kincaid

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
92 KB
Volume
258
Category
Article
ISSN
0003-2697

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## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %