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A Method for Isolation of Small DNA Fragments from Agarose and Polyacrylamide Gels

✍ Scribed by Jan Kormanec; Dagmar Homerova; Beatrica Sevcikova; Bronislava Rezuchova

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
63 KB
Volume
293
Category
Article
ISSN
0003-2697

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## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %