A freeze-squeeze method for recovering long DNA from agarose gels
β Scribed by R.W.J. Thuring; J.P.M. Sanders; P. Borst
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 455 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0003-2697
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A simple technique has been developed for eluting, in high concentration, macromolecular components separated in cylindrical tubes by gel electrophoresis. The principle can also be applied to the concentration of solutions of macromolecular components obtained through other means. Soluble complement
## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %