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An Improved Method for the Purification of Large DNA Fragments from Agarose Gels Using WizardPlusSV Columns

โœ Scribed by Daniel Tillett; Brett A. Neilan


Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
47 KB
Volume
269
Category
Article
ISSN
0003-2697

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## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %