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Adaptive weighted least squares method for the estimation of DNA fragment lengths from agarose gels

✍ Scribed by Akbar Akbari; Fritz Albregtsen; Ole Christian Lingjærde


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
783 KB
Volume
23
Category
Article
ISSN
0173-0835

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## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %