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Strand separation of DNA fragments and their isolation from nondenaturing polyacrylamide gels

✍ Scribed by Robert James; Ralph A. Bradshaw

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 388 KB
Volume: 140
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90193-3

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✦ Synopsis

A technique for rapidly and quantitatively denaturing double-stranded DNA employing urea and moderate heat is described. The single DNA strands are resolved on high-percentage nondenaturing polyacrylamide gels from which they can be recovered for Maxam-Gilbert sequence analysis.

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