## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %
A reliable method for the recovery of DNA fragments from agarose and acrylamide gels
โ Scribed by G. Dretzen; M. Bellard; P. Sassone-Corsi; P. Chambon
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 512 KB
- Volume
- 112
- Category
- Article
- ISSN
- 0003-2697
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A procedure for quick and simple elution of DNA from agarose gels is presented. After electrophoresis, bands of interest are cut out of the gel and the slices are equilibrated in a neutral salt buffer. The slices are then frozen and centrifuged through a filtration assembly whereby the DNA-containin
A simple and reliable method for the recovery of specific fragments of DNA from agarose gels is presented. The electroelution of the DNA onto the NENSORB cartridge matrix with the subsequent elution of the bound DNA by a methanol (50% v/v) wash has been shown to result in the quantitative recovery o