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Affinity chromatographic procedure for the quantitative recovery of DNA fragments from agarose gels

โœ Scribed by Maureen W. McEnery; C.William Angus; Joel Moss

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
605 KB
Volume
156
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A simple and reliable method for the recovery of specific fragments of DNA from agarose gels is presented. The electroelution of the DNA onto the NENSORB cartridge matrix with the subsequent elution of the bound DNA by a methanol (50% v/v) wash has been shown to result in the quantitative recovery of the restriction fragment. Of importance is the fact that the DNA purified by this procedure is a viable substrate for further digestion by a second restriction endonuclease. The method does not require either phenol extraction or extensive desalting of the sample.

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## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %