After electrophoresis, DNA can be efficiently recovered by solubilization of agarose gels with NaC104, followed by retention of DNA on glass fiber filters. After removal of the NaCIO, by ethanol, the DNA can be extracted with a low salt buffer.
Enhancing the sensitivity of DNA detection and recovery from agarose gels
โ Scribed by Fawzy Georges; Sophie Girard; Ravindra N. Chibbar; Friedrich Constabel
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 450 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %
A nontoxic and simple staining method for the detection of DNA in agarose gels is described. After eletrophoretic separation, the gels were stained with 5 g/ml of berberine (BB) prepared in distilled water and then the gels were soaked in 20 g/ml of aqueous Mordant Yellow 3R (MY3R) solution. Employm