Detection of DNA in Agarose Gels Using Berberine and Mordant Yellow 3R

✍ Scribed by Da-Woon Jung; Gyurng-Soo Yoo; Jung-Kap Choi

• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 91 KB
• Volume: 272
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1999.4198

✦ Synopsis

A nontoxic and simple staining method for the detection of DNA in agarose gels is described. After eletrophoretic separation, the gels were stained with 5 g/ml of berberine (BB) prepared in distilled water and then the gels were soaked in 20 g/ml of aqueous Mordant Yellow 3R (MY3R) solution. Employment of MY3R as a counterion dye efficiently quenched unwanted background fluorescence of BB. This method can detect as little as 10 ng of plasma membrane H ؉ -ATPase cDNA obtained from Arabidopsis thaliana L. (AHA1, 3.2 kb) under a long wavelength of UV irradiation (366 nm) within 1 h.