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Recovery of DNA segments from agarose gels

✍ Scribed by Carton W. Chen; Charles A. Thomas Jr.

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
186 KB
Volume
101
Category
Article
ISSN
0003-2697

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✦ Synopsis

After electrophoresis, DNA can be efficiently recovered by solubilization of agarose gels with NaC104, followed by retention of DNA on glass fiber filters. After removal of the NaCIO, by ethanol, the DNA can be extracted with a low salt buffer.

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## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %