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Transformation of rat and guinea-pig cells in vitro by SV40, and the transplantability of the transformed cells

✍ Scribed by Hans Diderholm; Rebekka Berg; Tore Wesslén

Publisher
John Wiley and Sons
Year
1966
Tongue
French
Weight
739 KB
Volume
1
Category
Article
ISSN
0020-7136

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✦ Synopsis

Cell cultures of rat andguinea-pig kidneys inoculated with simian virus 40 (SV40) were found to undergo morphological changes characteristic for S V40 transformation.

Cell lines of rapidly-growing transformed rat cells were obtained and found to be free from infectious SV40. They contained a specific antigen which was demonstrated in complement fixation tests with serum from hamsters bearing SV40 tumors.

When the transformed rat cells were injected subcutaneously into the autologous hosts, tumors histologically classified as sarcomas developed in animals which had been pretreated by X-ray irradiation. Tumor cells from one of the rats were passaged in vivo and gave rise to sarcomas of high malignancy also in non-treated animals. A tumor line was thus established in rats. It contained the specific complement-fixing '' tumor " antigen but no infectious SV40.

Transformed guinea-pig cells autotransplanted into the irradiated host caused a small tumor which regressed within a few weeks.

The results indicate that autotransplantation of in vitro-transformed cells into irradiated animals is a more promising way of obtaining SV40 tumors in diflerent animals than the inoculation of newborns with virus.

* Where no remarks are given, the animals were killed when moribund.

** Figures within brackets indicate

days in tissue culture after transformation. *** Minced tumor tissue was transplanted.

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