Transformation of hamster cells in vivo and in vitro by an adenovirus 4-SV40 hybrid

✍ Scribed by John M. Easton; Alan S. Rabson; Richard A. Malmgren

• Publisher: John Wiley and Sons
• Year: 1970
• Tongue: French
• Weight: 639 KB
• Volume: 5
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910050103

✦ Synopsis

Abstract

A preparation of adenovirus 4, containing infectious SV40 genetic material enclosed within adenovirus coats (Ad. 4‐SV40 hybrid), induced both transformation of newborn hamster kidney cells in vitro and tumors in neonatally inoculated hamsters. Injection of transformed cells into an irradiated weanling hamster also resulted in a tumor. Histologically, all of the tumors were fibrosarcomas similar to those induced in vivo by SV40 alone; no epithelial elements were noted. The three tumors were carried as serial transplants in hamsters; each of the three lines maintained a morphology slightly different from that of the other two. Sera from hamsters carrying the tumors contained antibodies to T antigens induced in cell cultures of African green monkey kidney by Ad. 4 and Ad. 7, but not by Ad. 12. This confirmed work by others, working with Ad. l‐SV40 and Ad. 2‐SV40 hybrid viruses, who found that genetic material from nononcogenic adenoviruses can be introduced into tumors and transformed cells by these hybrids. The cross‐reaction with cells infected with Ad. 7 provides additional evidence for a relationship between Ad. 4 and the B subgroup of oncogenic adenoviruses.