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Differences in the manifestation of virus-specific surface antigen between cells transformed by sv40 and uv-irradiated sv40

✍ Scribed by W. Klietmann; N. Seemayer

Publisher
John Wiley and Sons
Year
1971
Tongue
French
Weight
729 KB
Volume
7
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Marked differences in the manifestation of SV40‐specific surface (S‐) antigen were found between hamster cell lines transformed in vitro and in vivo by SV40 and UV‐irradiated or photodynamically inactivated SV40. In the mixed hemagglutination reaction (MHA) cell lines induced by non‐irradiated SV40 yielded positive results when tested against specific antisera up to dilutions of 1:1280. In comparison, cell lines induced by inactivated SV40 only showed positive reactions in the MHA‐test in dilutions up to 1:80 or 1:160 respectively. The demonstration of small quantities of SV40‐specific S‐antigen in cell lines transformed by inactivated SV40 could only be achieved by use of hyperimmune sera produced by a special immunization procedure. However, if these cells were tested in the MHA reaction against anti‐S‐sera, produced according to conventional methods described in the literature, they yielded negative results. All cell lines with diminished quantities of demonstrable S‐antigen caused malignant tumors when inoculated into adult hamsters. The capacity of UV‐irradiated SV40 to induce a specific transplantation resistance in adult Syrian hamsters was investigated. The irradiated virus induced a markedly reduced transplantation resistance.

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