𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detection of specific surface antigens by colony inhibition in cells transformed by papovavirus SV40

✍ Scribed by Satvir S. Tevethia; Norman A. Crouch; Joseph L. Melnick; Fred Rapp

Publisher
John Wiley and Sons
Year
1970
Tongue
French
Weight
661 KB
Volume
5
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Specific antigens at the surface of SV40‐transformed hamster cells were demonstrated by the in vitro colony inhibition and immunofluorescence tests. Antisera were prepared by inoculating hamsters either with purified SV40 or with human or marmoset SV40‐transformed cells. The inhibition of colony formation by the transformed cells ranged from 46 to 100% in the presence of specific antibody and complement. Nontransformed hamster cells were not inhibited by the immune sera.

Of the 22 sera tested, 12 were positive in the colony inhibition test and 14 were positive in the immunofluorescence test. Eleven of the 22 sera tested were positive in both tests. This positive correlation between the colony inhibition and immunofluorescence tests suggests that the two tests are detecting the same or similar antigens.

📜 SIMILAR VOLUMES

Evidence for virus-specific transplantat
Evidence for virus-specific transplantation antigen in cells of lymphoid neoplasms induced by papovavirus sv40
✍ S. S. Tevethia 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 430 KB 👁 1 views

## Abstract Hamster lymphocyte leukemia and lymphosarcoma induced in vivo __by papova‐virus SV40 were tested for the presence of SV40‐specific transplantation antigen. The cells derived from lymphocytic leukemia (GD‐248) blocked primary SV40 oncogenesis__ in vivo and also immunized adult hamsters a

Differences in the manifestation of viru
Differences in the manifestation of virus-specific surface antigen between cells transformed by sv40 and uv-irradiated sv40
✍ W. Klietmann; N. Seemayer 📂 Article 📅 1971 🏛 John Wiley and Sons 🌐 French ⚖ 729 KB

## Abstract Marked differences in the manifestation of SV40‐specific surface (S‐) antigen were found between hamster cell lines transformed __in vitro__ and __in vivo__ by SV40 and UV‐irradiated or photodynamically inactivated SV40. In the mixed hemagglutination reaction (MHA) cell lines induced by

New antigens in cells transformed by the
New antigens in cells transformed by the SV40 virus. III. Presence of an SV40-coded antigen on the surface of different cell lines transformed by this virus
✍ A. Duthu-Herbet; M. Kress; Ch. De Vaux Saint Cyr 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 French ⚖ 669 KB

## Abstract The sera of hamsters carrying tumours induced by injection of TSV~5~Cl~2~ cells and the sera of animals immunized with these cells or with SV40 itself or its purified capsids have a slow cytotoxicity activity in the presence of fresh guinea pig complement on SV40‐transformed cell strain

In vivo induction of tumor-specific immu
In vivo induction of tumor-specific immunity by glycolipid extracts of SV40-transformed cells
✍ Sandra Ansel; Daniel Blangy 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 French ⚖ 482 KB 👁 1 views

Glycolipid extracts were prepared from various Syrian golden hamster cell lines, either SV40-transformed or spontaneously transformed. To detect possible SV40-TSTA activity of the glycolipid preparations. normal hamsten were inoculated with different glycolipid extracts and were subsequently challen

Inhibition of initiator-induced SV40 gen
Inhibition of initiator-induced SV40 gene amplification in SV40-transformed Chinese hamster cells by infection with a defective parvovirus
✍ Jörg R. Schlehofer; Regine Heilbronn; Brigitte Georg-Fries; Harald Zur Hausen 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 French ⚖ 469 KB 👁 1 views

## Abstract Amplification of SV40 genes in SV40‐transformed Chinese hamster embryo cells (CO631) by chemical carcinogens as well as by herpes simplex virus infection can be inhibited by infection with the defective parvovirus, AAV‐5. This is shown by __in situ__ hybridization with SV40 DNA of AAV‐5

New antigens in cells transformed by the
New antigens in cells transformed by the SV40 virus. II. Evidence of their cytoplasmic localization in a cell line (TSV5 CL2) of hamster fibroblasts transformed by SV40
✍ G. P. Tilz; Ch. De Vaux Saint Cyr; P. Grabar 📂 Article 📅 1969 🏛 John Wiley and Sons 🌐 French ⚖ 606 KB

A clone of hamster fibroblasts tramformed by SV40 virus (TSVKCL,) induced antibodies in hamsters against new antigenic constituents not present in the untransformed fibroblasts. The antibodies in the immune sera against histocompatibility antigens and those against nuclear T antigen were absorbed. T