𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Synthesis of fluorescent and radiolabeled analogues of phosphatidic acid

✍ Scribed by Kenneth J. Longmuir; Ona C. Martin; Richard E. Pagano

Book ID
103038731
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
572 KB
Volume
36
Category
Article
ISSN
0009-3084

No coin nor oath required. For personal study only.

✦ Synopsis

Procedures tbr the synthesis of tluorescent and radiolabeled analogues of phosphatidic acid arc described. The |luorophore 7-nitrobenzo-2.-oxa-l,3-diazole (NBD) was coupled to 6-aminocaproic acid and 12-aminododet~noic acid by reaction of NBD-chloride with the amin~ acids under mild alkaline conditions at room temperature. 1,2-Dioleoyl-sn-iU-t'~('Jglycerol 3-phosphate was prepared by acylation of sn-lU-~4C]glycerol 3-phosphate with oleic acid anhydridc using dimcthylaminopyridine as the ~talyst. This compound was converted to l-oleoyl-sn-[U-~4(']glycerol 3-phosphate by hydrolysis with phospholipase A a. The lysophosphatidic acid was reacylated with NBD-aminocaproyl imidazole or NBD-aminododecanoyl imidazol~ to form the t]uorescent, radiolabeed analogue of phospbatidic acid. Fluoresoent, non-radiolabeled analogues of phosphatidic acid were prepared by phospholipasc D hydrolysis of fluorescent phosphatidylcholine.

📜 SIMILAR VOLUMES

Synthesis of radiolabelled photolabile f
Synthesis of radiolabelled photolabile fusidic acid analogues
✍ Søren Christian Schou; Ditte Riber; Gunnar Grue-Sørensen 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 French ⚖ 107 KB

## Abstract Two analogues of the antibiotic fusidic acid with photolabile groups, 4‐azidophenyl and 4‐benzoylphenyl, were successfully labelled with tritium via Pd/C catalysed tritiation of unsaturated precursors. Specific activities of 36 and 44 Ci/mmol were obtained. Copyright © 2007 John Wiley &

Synthesis of radiolabelled clenbuterol a
Synthesis of radiolabelled clenbuterol analogues
✍ Graham G. Pegg; Martin N. Sillence; Madonna J. Sleeman; Derek B. Lindsay 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 French ⚖ 327 KB

## Abstract Oxidation of clenbuterol 1a with pyridinium chlorochromate yielded 4‐amino‐3,5‐dichloro‐α‐__tert__‐butylaminoacetophenone 5. Tritiated clenbuterol 1b was produced by reduction of 5 with sodium [^3^H]borohydride. Radioiodination of the clenbuterol precursor [2‐__tert__‐butylamino‐1‐(4‐am

Phosphonolipids. The synthesis of chiral
Phosphonolipids. The synthesis of chiral vinylic phosphonolipid analogues of phosphatidic acid and phosphatidyl choline
✍ Patricia Waters Schwartz; Burton E. Tropp; Robert Engel 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 532 KB

A series of phosphonic acid analogues of phosphatidic acid and phosphatidyl choline have been synthesized in which an olefinic linkage has been incorporated into the backbone portion of the molecule. These analogues bear a structural rigidity not available with simple isosteric or non-isosteric phos

Facile synthesis of novel fluorescent di
Facile synthesis of novel fluorescent distamycin analogues
✍ Santanu Bhattacharya; Mini Thomas 📂 Article 📅 2001 🏛 Elsevier Science 🌐 French ⚖ 200 KB

A facile synthesis of four distamycin analogues that bear the dansyl fluorophore is described. The nature of the linkage between the fluorophore and the sequence recognition element had a dramatic effect on the fluorescence properties of these ligands upon DNA binding.