Peptide nucleic acids (PNA) were synthesized by a modified Merrifield method using several improvements. Activation by O-[benzotriazol-1-yl]-1,1,3,3-tetramethyluronium hexafluorophosphate in combination with in situ neutralization of the resin allowed efficient coupling of all four Boc-protected PNA
Solid-phase synthesis of pseudo-complementary peptide nucleic acids
โ Scribed by Komiyama, Makoto; Aiba, Yuichiro; Ishizuka, Takumi; Sumaoka, Jun
- Book ID
- 109946619
- Publisher
- Nature Publishing Group
- Year
- 2008
- Tongue
- English
- Weight
- 261 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1750-2799
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๐ SIMILAR VOLUMES
Boc/'Z-protected PNA oligomers were synthesised on solid phase. The use of the allylic HYCRON resin allowed for the application of both Boc-and Fmoc-protecting groups. Highest yields were obtained when the monomeric building block was synthesised on solid phase rather than loaded as preformed unit.
The first solid-phase synthesis of novel peptide-derived enantiospecific nucleic acid analogs (PDNAs), employing all three stereoisomers of 4-(N 9 -adeninyl)-2-amino(t-boc)-butyric acid as ADNA (amino acid-derived nucleoside analogs) monomers has been described. The preliminary melting temperature (
Solid phase synthesis of a nucleic acid analog peptide (NAAP), as a substitute for antisense or triple-helix forming oligonucleotide, is described. The N-protected amino acid monomer was prepared starting from 3'-azido-3'-deoxythymidine (AZT) in 20 % overall yield. Condensation of the amino acid mon