Peptide nucleic acids (PNA) were synthesized by a modified Merrifield method using several improvements. Activation by O-[benzotriazol-1-yl]-1,1,3,3-tetramethyluronium hexafluorophosphate in combination with in situ neutralization of the resin allowed efficient coupling of all four Boc-protected PNA
Solid phase synthesis of a self complementary (antiparallel) chiral peptidic nucleic acid strand
β Scribed by Alessandro Lenzi; Gianna Reginato; Maurizio Taddei; Elisabeth Trifilieff
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- French
- Weight
- 110 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0040-4039
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Boc/'Z-protected PNA oligomers were synthesised on solid phase. The use of the allylic HYCRON resin allowed for the application of both Boc-and Fmoc-protecting groups. Highest yields were obtained when the monomeric building block was synthesised on solid phase rather than loaded as preformed unit.
Solid phase synthesis of a nucleic acid analog peptide (NAAP), as a substitute for antisense or triple-helix forming oligonucleotide, is described. The N-protected amino acid monomer was prepared starting from 3'-azido-3'-deoxythymidine (AZT) in 20 % overall yield. Condensation of the amino acid mon
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