Liquid phase synthesis of a peptidic nucleic acid dimer
✍ Scribed by Audrey Farèse; Nadia Patino; Roger Condom; Sandrine Dalleu; Roger Guedj
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- French
- Weight
- 211 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
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Suitably protected linear precursors of cyclic PNAs can be readily obtained by BOP/DiPEA coupling of the corresponding sub-monomers. Conversion of the linear PNA dimers into the pentafluorophenyl esters allows cyclization by intramolecular attack of the deprotected primary amino function under dilut
Peptide nucleic acids (PNA) were synthesized by a modified Merrifield method using several improvements. Activation by O-[benzotriazol-1-yl]-1,1,3,3-tetramethyluronium hexafluorophosphate in combination with in situ neutralization of the resin allowed efficient coupling of all four Boc-protected PNA