Synthesis of peptide nucleic acids (PNA) by submonomer solid-phase synthesis
โ Scribed by Lutz S. Richter; Ronald N. Zuckermann
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 250 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0960-894X
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Boc/'Z-protected PNA oligomers were synthesised on solid phase. The use of the allylic HYCRON resin allowed for the application of both Boc-and Fmoc-protecting groups. Highest yields were obtained when the monomeric building block was synthesised on solid phase rather than loaded as preformed unit.
A new simple solid-phase method has been developed for synthesizing Boc-protected peptide nucleic acid (PNA) monomers. An immobilized backbone 3 was built on Expansin ยฎ resin using an ester disulphide handle: 2-hydroxypropyl-dithio-2%-isobutyric acid (HPDI). The base acetic acids of thymine 5, Z-cyt
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