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Solid-phase hydrolysis of the p-nitroanilide of N-succinyl-L-phenylalanine in the presence of chymotrypsinogen

✍ Scribed by N. V. Medvedeva; Yu. I. Khurgin


Book ID
112438574
Publisher
Springer
Year
1977
Tongue
English
Weight
120 KB
Volume
26
Category
Article
ISSN
1573-9171

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A calorimetric assay for the measurement of cathepsin B using N-benzoyl-r.-phenylalanyl-L-valyl+arginine-p-nitroanilide (S2160) as substrate has been developed. The technique is reproducible and simpler, quicker, safer, and more sensitive than that using cr-N-benzoyl-DL-arginine-P-naphthylamide as s