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Substrate activation in the trypsin-catalyzed hydrolysis of benzoyl-L-arginine p-nitroanilide

✍ Scribed by Hiroyasu Nakata; Shin-Ichi Ishii


Book ID
118851845
Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
395 KB
Volume
41
Category
Article
ISSN
0006-291X

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A calorimetric assay for the measurement of cathepsin B using N-benzoyl-r.-phenylalanyl-L-valyl+arginine-p-nitroanilide (S2160) as substrate has been developed. The technique is reproducible and simpler, quicker, safer, and more sensitive than that using cr-N-benzoyl-DL-arginine-P-naphthylamide as s