✦ LIBER ✦
The hydrolysis of N-benzoyl-l-phenylalanyl-l-valyl-l-arginine-p-nitroanilide and its use as a substrate for the assay of cathepsin B
✍ Scribed by John Butterworth; Judith J. Duncan
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 428 KB
- Volume
- 106
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A calorimetric assay for the measurement of cathepsin B using N-benzoyl-r.-phenylalanyl-L-valyl+arginine-p-nitroanilide (S2160) as substrate has been developed. The technique is reproducible and simpler, quicker, safer, and more sensitive than that using cr-N-benzoyl-DL-arginine-P-naphthylamide as substrate. The assay has been used to measure cathepsin B in human skin fibroblasts, amniotic fluid cells, and autopsy tissues. The greatest activity was found in the thyroid.