l-Pyroglutamyl-l-phenylalanyl-l-leucine-
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I.Yu. Filippova; E.N. Lysogorskaya; E.S. Oksenoit; G.N. Rudenskaya; V.M. Stepano
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Article
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1984
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Elsevier Science
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English
⚖ 354 KB
L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA)--a convenient chromogenic substrate for assay of thiol proteinases papain, ficin, and bromelain--was prepared by enzymatic synthesis with chymotrypsin as a catalyst. The thiol proteinases hydrolyze PFLNA with the liberation of p-nitroani