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A chromogenic substrate ofα-chymotrypsin — The p-nitroanilide of L-pyroglutamyl-L-phenylalanine

✍ Scribed by E. S. Oksenoit; E. N. Petrova; I. Yu. Filippova; G. I. Lavrenova; N. I. Tarasova; V. M. Stepanov


Book ID
104817803
Publisher
Springer
Year
1983
Tongue
English
Weight
101 KB
Volume
19
Category
Article
ISSN
0009-3130

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l-Pyroglutamyl-l-phenylalanyl-l-leucine-
✍ I.Yu. Filippova; E.N. Lysogorskaya; E.S. Oksenoit; G.N. Rudenskaya; V.M. Stepano 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 354 KB

L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA)--a convenient chromogenic substrate for assay of thiol proteinases papain, ficin, and bromelain--was prepared by enzymatic synthesis with chymotrypsin as a catalyst. The thiol proteinases hydrolyze PFLNA with the liberation of p-nitroani