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Hydrolysis of N-α-benzoyl-L-phenylalanyl-L-valyl-L-arginine-p-nitroanilide by human alpha thrombin in the presence of heparin

✍ Scribed by Michael J. Griffith; Henry S. Kingdon; Roger L. Lundblad


Book ID
119034362
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
599 KB
Volume
17
Category
Article
ISSN
0049-3848

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The hydrolysis of N-benzoyl-l-phenylalan
✍ John Butterworth; Judith J. Duncan 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 428 KB

A calorimetric assay for the measurement of cathepsin B using N-benzoyl-r.-phenylalanyl-L-valyl+arginine-p-nitroanilide (S2160) as substrate has been developed. The technique is reproducible and simpler, quicker, safer, and more sensitive than that using cr-N-benzoyl-DL-arginine-P-naphthylamide as s