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Sedimentation coefficients of replicating and crosslinked DNA

✍ Scribed by Victor A. Bloomfield

Publisher: Wiley (John Wiley & Sons)
Year: 1968
Tongue: English
Weight: 415 KB
Volume: 6
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1968.360060303

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✦ Synopsis

The sedimentation coefficients of branched and cyclic replicating DNA structures have been calculated taking into account both chain stiffness and excluded volume effects. For branched DNA, the calculated ratio So(a)/SO(0) of the sedimentation coefficient at degree of replication a to that a t a = 0 increases from 1.000 to 1.331 as a goes from 0 to 1.

The considerable difference between these two ratios for a = 1 suggests that the two types of replivating structures may feasibly be distinguished by sedimentation velocity studies, A similar calculation has been made of the sedimentation coefficient of singly crosslinked UNA under denaturing conditions, as a function of placement of the crosslink. In 0.195M Naf, the ratio So(a)/So(o) varies from 1.000 a t a = 0 to 1.124 a t a = 0.5, where a is the fractional distance of the crosslink from the end of the chain.

For cyclic replicating DNA, So(l)/SO(0) = 1.517.

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