The sedimentation coefficient of T2 phage DNA has been studied by zone centrifugation in sector-shaped preparative centrifuge tubes over a concentration range of 0.02-2.0 rg./ml. DNA. These results have been compared to a similar study in the analytical centrifuge of T4 DNA over the range of 0.50-5.
Sedimentation coefficient of polyoma virus DNA
β Scribed by Horace B. Gray Jr.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1967
- Tongue
- English
- Weight
- 557 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
β¦ Synopsis
The sedimentation coefficient of the twisted circular form of polyoma virus DNA is calculat,ed from the Kirkwood sedimeiit,ation-diffrision eqiiat ion, the strrictiire being assumed to be a rigid donble superhelix. Agreement with the experimental sedimentation coefficient can be obt,ained, with the use of an experimental valiie for the number of SIIperhelical turns, when t,he pit,ch of the siiperhelix is intermediate between its minimal and maximal possible values. Another model, which has been proposed for polyoma DNA at low ionic strengths, may be visualized as a superhelical stxuctjrire woiind about a t~orns. Calculations of sedimentation coefficienk for this model agree qualitatively witth experiment,al dat2a at. ionic strengt,hs below 10-*M.
π SIMILAR VOLUMES
The sedimentation coefficients of branched and cyclic replicating DNA structures have been calculated taking into account both chain stiffness and excluded volume effects. For branched DNA, the calculated ratio So(a)/SO(0) of the sedimentation coefficient at degree of replication a to that a t a = 0
## Synopsis The sedimentation coefficient sN of the DNA topoisomer with the linking number N is evaluated as a function of N and chain length on the basis of a (circular) twisted wormlike chain, i.e., a special case of the helical wormlike chain. Evaluation is carried out by an application of the
## Abstract Standardization in the calculation of the sedimentation coefficient of macromolecules by means of band techniques is discussed. When a sample of macromolecules suspended in a solvent is layered on to a denser bulk solution, the macromolecules do not sediment in this solution alone, but
## Abstract Polyomaβvirus (PV)βtransformed cell clones, which are inducible for virus synthesis by various physical and chemical agents, metabolize the chemically nonβreactive carcinogen benzo (a)pyrene (BP) into waterβsoluble products. In cultures of such clones, which metabolize BP to a level of