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Scale-up of dialysis fermentation for high cell density cultivation of Escherichia coli

✍ Scribed by C Fuchs; D Köster; S Wiebusch; K Mahr; G Eisbrenner; H Märkl

Book ID
119524129
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
177 KB
Volume
93
Category
Article
ISSN
0168-1656

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✍ Dieter Riesenberg 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 585 KB

High-cell-density cultivations of Escherichia coil in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.