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High cell density cultivation of recombinant Escherichia coli for hirudin variant 1 production

โœ Scribed by Toru Matsui; Hiroaki Sato; Hotaka Yamamuro; Satoru Misawa; Naoya Shinzato; Hitoshi Matsuda; Joji Takahashi; Seigo Sato

Book ID
118472584
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
370 KB
Volume
134
Category
Article
ISSN
0168-1656

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โœ Dieter Riesenberg ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 585 KB

High-cell-density cultivations of Escherichia coil in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.