Synthesis of an operon fusion protein was investigated in batch and fed-batch cultures at high cell densities of recombinant Escherichia coli JM105 [pBAD-GFP::CAT]. Glucose-limited growth was achieved without accumulation of inhibitory byproducts allowing high cell densities (110 g L -1 DCW) to be a
β¦ LIBER β¦
High-cell-density cultivation of Escherichia coli
β Scribed by Dieter Riesenberg
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 585 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0958-1669
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β¦ Synopsis
High-cell-density cultivations of Escherichia coil in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.
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