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Simple fed-batch technique for high cell density cultivation of Escherichia coli

✍ Scribed by D.J. Korz; U. Rinas; K. Hellmuth; E.A. Sanders; W.-D. Deckwer

Book ID
118034416
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
647 KB
Volume
39
Category
Article
ISSN
0168-1656

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High-cell-density cultivations of Escherichia coil in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.