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High cell density cultivation of Escherichia coli at controlled specific growth rate

✍ Scribed by D. Riesenberg; V. Schulz; W.A. Knorre; H.-D. Pohl; D. Korz; E.A. Sanders; A. Roß; W.-D. Deckwer

Book ID
118010684
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
634 KB
Volume
20
Category
Article
ISSN
0168-1656

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High-cell-density cultivation of Escherichia coli
✍ Dieter Riesenberg 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 585 KB

High-cell-density cultivations of Escherichia coil in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.