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Control of Escherichia coli growth rate through cell density

✍ Scribed by Xavier Carbonell; José Luis Corchero; Rafael Cubarsí; Pau Vila; Antonio Villaverde

Book ID
114494022
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
105 KB
Volume
157
Category
Article
ISSN
0944-5013

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High-cell-density cultivations of Escherichia coil in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.