𝔖 Bobbio Scriptorium
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Application of an expert system to high cell density cultivation of Escherichia coli

✍ Scribed by Michimasa Kishimoto; Hideyuki Suzuki

Book ID
113397718
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
608 KB
Volume
80
Category
Article
ISSN
0922-338X

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High-cell-density cultivations of Escherichia coil in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.