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RAPID RECOVERY OF DNA FROM AGAROSE GEL SLICE USING A MICROWAVE

โœ Scribed by XUEZHI OUYANG; WENWEN YU; QIANG WANG; JIANGUO HE; QIUYUN LIU

Book ID
111344519
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
113 KB
Volume
14
Category
Article
ISSN
1060-3999

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๐Ÿ“œ SIMILAR VOLUMES

Recovery of DNA segments from agarose ge
Recovery of DNA segments from agarose gels
โœ Carton W. Chen; Charles A. Thomas Jr. ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 186 KB

After electrophoresis, DNA can be efficiently recovered by solubilization of agarose gels with NaC104, followed by retention of DNA on glass fiber filters. After removal of the NaCIO, by ethanol, the DNA can be extracted with a low salt buffer.

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We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 an